单细胞测序文库构建系统
发布时间:2021-04-13 16:12:00

单细胞测序文库构建系统

Single Cell Sequencing Library Generate System

型号:Chromium Single Cell Controller & Accessory Kit


介绍:

10x Genomics这款基于微流控技术的单细胞系统,通过序列标签区别群体中的不同细胞,获得单细胞水平的数字化基因表达谱,可实现数千甚至数万个单细胞群体分析,实现对细胞群体的划分与细胞群体间基因表达差异的检测,解决常规scRNA-seq方法在通量或扩展性方面存在的不足,为单细胞研究开拓新的思路。是肿瘤细胞异质性、免疫细胞群体检测以及胚胎发育研究的黄金方法。

This 10 x Genomics single-celled system based on microfluidic technology, through the sequence tags difference between groups of different cells, get the single-cell level digital gene expression profile, which can realize thousands or even tens of thousands of single cell group analysis, implementation of group of cell division and cell detection, gene expression differences between conventional scRNA - seq solution in flux or extensibility of open up some new thoughts for the study of the single cell. It is a golden method for the study of tumor cell heterogeneity, immune cell population and embryo development.

技术参数:


1. 每次运行处理8个样品,每个样品可以收集100-10000个细胞

2. 每次细胞分装运行时间不长于10分钟,整个样品制备时间不长于8小时;

3. 细胞捕获效率>60%;

4. 测序结果中doublet比例每1000个细胞不大于1%;

5. 提供大于100万个独立反应体系;

6. 提供大于400万种标签序列,确保每个液体体系均可以获得唯一的标记

7. 系统提供Illumina兼容测序文库


用途:

单细胞测序文库构建,测序